Testosterone, Total, Males (Adult), Immunoassay

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Testosterone, Total, Males (Adult), Immunoassay


Test Description

Also known as Total Testosterone Test or Bioavailable Testosterone, this test is done to detect abnormalities in the Testosterone level in both males and females. In males, the diagnosis helps in finding the cause of erectile dysfunction or infertility issues. In females, the test helps to diagnose virilization (cause of masculine physical features), infertility, or PCOD. In children, the test helps in determining the cause of genitals that are hard to be categorized in males or females.

What is being tested and how the test is used?
Testosterone is a sex hormone that is responsible for male characteristics. Mostly, the hormone is considered a male hormone, but it is present in both men and women. The test will measure the Testosterone level in blood. The hormone is mainly produced in male testicles by endocrine tissues. The adrenal gland also produces this hormone in both males and females and a minimal amount in women ovaries. In general cases, the measurement of total testosterone in the body provides the healthcare provider adequate information of the bodily function. In some instances, where the level of SHBG (sex hormone-binding globulin) is abnormal, this test is recommended.

The Testosterone levels in the body help to evaluate specific conditions like:

  • Delayed or early puberty in boys
  • Decreased sex drive in both men and women
  • Erectile dysfunction in men
  • Infertility for both men and women
  • Testicular tumors in men
  • Pituitary disorder or hypothalamus
  • Hirsutism and virilization in women
  • The total testosterone test measures the Testosterone that is not bound and the Testosterone, which is bound to blood proteins such as albumin or SHBG. Depending on the reasons why the doctors suggested this test for you, other tests might also be done alongside such as SHBG, FSH, and LH, Prolactin, Estrogen, DHEAS, etc.

    When is the Bioavailable Testosterone Test Ordered?
    In men, the test is ordered mostly to check infertility that is associated with decreased sex drive or erectile dysfunction. Low levels of total testosterone are also associated with visceral fat, insulin resistance, or coronary artery disease. In women, this test is done when the menstrual periods are irregular or no menstrual periods at all. This test is ordered when there is a difficulty in getting pregnant, and the woman seems to have masculine features. The levels can also ascend if there is a tumor developing in the ovaries or adrenal glands in women.


    In men, the increased Testosterone levels indicate:
  • Testicular tumors
  • Adrenal tumors
  • Androgen use also known as anabolic steroids
  • Early puberty without an unknown cause
  • Congenital adrenal hyperplasia in babies

  • In females, the high Testosterone levels are a sign of:
  • Polycystic Ovarian Syndrome
  • Ovarian or Adrenal gland tumor
  • Congenital Adrenal Hyperplasia

  • While in general, women have a very low level of Testosterone, there are no more significant risks. However, in men, a low level of Testosterone is a severe condition that can be treated by Testosterone Replacement therapy.

    Testing Info

    Alternate Name: Total Testosterone Male Blood Test

    Test Instructions : Due to changes in testosterone levels throughout the day, two morning (8:00-10:00 a.m.) specimens obtained on different days are recommended by The Endocrine Society for screening. Please do not exercise prior to testing. It is best that your blood is taken in a rested state.

    Fasting: No

    Methodology: Immunoassay (IA)

    Results In: 1 Working Day

    Note: Turnaround times on results are an estimate and are not guaranteed. The lab may need additional time due to holidays, confirmation/repeat testing, etc. You can contact us to discuss when your results should be ready and we will contact the lab on your behalf immediately.